Incucyte® Kinase Akt Green/Red Lentivirus

The Incucyte^® Kinase Akt Green/Red Lentivirus enables efficient, non-perturbing, and homogenous labeling of mammalian cells for in vitro analysis of dynamic changes in Akt kinase activity. The lentivirus reagent is a single-cassette, genetically encoded sensor based on a green fluorescent protein (TagGFP2)-tagged Akt substrate whose subcellular localization is phosphorylation-dependent, and a red fluorescent protein (mKate2) nuclear marker to denote the nuclear/cytoplasmic boundary. Upon phosphorylation by Akt, the green fluorescent sensor will translocate from the nucleus to the cytoplasm. Inhibition of Akt will prevent phosphorylation of the sensor and cause it to be retained in the nucleus. It is driven by an EF-1α promotor with puromycin selection to allow generation of cell lines or clones which stably express the genetically encoded dual fluorescent (TagGFP2 and mKate2) indicator.

The Incucyte^® Kinase Akt Green/Red Lentivirus has been validated for use with the Incucyte^® SX5, S3, or SX1 Live-Cell Analysis System configured with a Green/Red Optical Module. It is not compatible with Incucyte instruments configured with a Green/Orange/NIR or an Orange/NIR Optical Module.