CIMac™ QA 0.1 mL Analytical Column (1.3 µm)

Tech Support Documents
CIMac™ QA 0.1 mL Analytical Column (1.3 µm)
 

This high-performance analytical column is primarily intended for fast, efficient and reproducible analysis and in-process control of proteins, viral particles and other biomolecules under hydrophilic conditions throughout different purification steps.

Item No.: 
110.5113-1.3
Piece
1

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CIMac™ Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short column length allow the operation at high volumetric flow rates, enabling them to receive the information about the product quantity and purity in just a few minutes. Hence, the CIMac™ Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps.

CIMac™ OH Analytical column are primarily intended for fast, efficient and reproducible analysis and in-process control of proteins, viral particles and other biomolecules under hydrophilic conditions throughout different purification steps.

OH (hydroxyl) features:

  • Uncharged (neutral) ligand
  • Working range, pH 2–10
  • Retains very large solutes (virus particles, VLP, vesicles) in the presence of precipitating salts or polyethylene glycol, and elutes them with high resolution in order of increasing size
  • Elution with descending salt or polyethylene glycol
  • Sanitizable with 1 M NaOH

Applications

  • Application
    Analytical Columns  

Connections (Physical)

  • Connectors
    10-32 UNF coned port, 0.0625" OD tubing connection  

Dimensions

  • Channel Size
    1.3 µm  
  • Diameter
    5.2 mm  
  • Length
    4.95 mm  

Features

  • Reusable
    Yes  

General Specifications

  • Flow Rate
    0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min  

Materials of Construction

  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)  

Operating Conditions

  • Maximum Operating Pressure
    15 MPa | 150 bar | 2175 psi  
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)  
  • pH Stability
    Working range 2–13, cleaning in place 1–14  

Physicochemical Information

  • Bed Volume
    0.1 mL  
  • Ligand
    QA (strong anion exchanger, quaternary amine)  
  • Ligand Density
    0.45–0.65 mmol/mL wet support  
  • Typical Dynamic Binding Capacity 10% per mL
    25–31 mg BSA/mL wet support, BSA, 0.5 mg/mL, 20 mM Tris-HCI buffer, pH 7.4, flowrate 1 mL/min  

Product Information

  • Brand
    CIMac™