CIMac™ r-Protein A 0.1 mL Analytical Column (2 µm)

Tech Support Documents
CIMac™ r-Protein A 0.1 mL Analytical Column (2 µm)
 

CIMac™ r-Protein Analytical Columns are primarily intended for fast, efficient, and reproducible analysis of immunoglobulin G (IgG).

Item No.: 
110.1004-2
Piece
1

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CIMac™ Analytical Columns are short-bed high-performance monolithic columns offering all the advantages of CIM® monolithic technology. Their small volume and short column length allow the operation at high volumetric flow rates, enabling them to receive the information about the product quantity and purity in just a few minutes. Hence, the CIMac™ Analytical Columns can be effectively used for the in-process and final control of various samples from different purification process steps.

CIMac™ r-Protein Analytical Columns are primarily intended for fast, efficient, and reproducible analysis of immunoglobulin G (IgG) from various samples. They can be effectively used for in-process and final control (quantitative and qualitative) of IgG purified from cell culture supernatant or human plasma. The innovative stainless steel housing allows flow in both directions, increasing its durability and ease of use.

rProtein A (recombinant protein A from E. coli) features:

  • Used for purification of mouse and most subtypes of human immunoglobulin G (IgG)
  • Primary binding to the hinge region of IgG-Fc of IgG, secondary binding to light chains of other antibody classes
  • Most suitable for purification of human IgG, and many IgM monoclonal antibodies

Applications

  • Application
    Analytical Columns  

Connections (Physical)

  • Connectors
    10-32 UNF coned port, 0.0625" OD tubing connection  

Dimensions

  • Channel Size
    2 µm  
  • Diameter
    5.2 mm  
  • Length
    4.95 mm  

Features

  • Reusable
    Yes  

General Specifications

  • Flow Rate
    0.2 – 3 mL/min | 1 – 15 cm/min | 2 – 30 CV/min  

Materials of Construction

  • Materials of Construction
    Poly(glycidyl methacrylate -co- ethylene dimethacrylate)  

Operating Conditions

  • Maximum Operating Pressure
    15 MPa | 150 bar | 2175 psi  
  • Recommended Operating Temperature
    4 °C (39 °F) to 40 °C (104 °F)  
  • pH Stability
    Working range 2–11, cleaning in place 2–13  

Physicochemical Information

  • Bed Volume
    0.1 mL  
  • Ligand
    r-Protein A (affinity, recombinant protein A produced from E. Coli coupled to CDI-preactivated support)  
  • Typical Dynamic Binding Capacity 10% per mL
    ≥ 5 mg hIgG/mL wet support, human IgG 0.5 mg/mL, PBS buffer, pH 7.4, flow rate 1mL/min, Human IgG: octagam® 5% (Octapharma®)  

Product Information

  • Brand
    CIMac™