CIMmultus PrimaS® HR 1 mL Monolithic Column (2 μm)

CIMmultus^® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.

They can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.

CIMmultus PrimaS® HR is a monolithic chromatographic multimodal column that combines elements of hydrogen bonding with weak AEX. PrimaS® HR provides consistent column performance, significantly enhancing the consistency of purity and recovery at any scale. When reproducible chromatography is crucial, for example AAV empty - full separation, PrimaS HR is the recommended choice.

PrimaS® HR features:

• Multimodal chromatography ligand that combines elements of hydrogen bonding with weak anion exchange chromatography.
• Selected to meet the need for highly reproducible purification processes (e.g., separation of full AAV capsids from empty AAV capsids)
• Column release criteria based on the AAV8 elution profile.
• AAV Binding at neutral or closer to neutral pH compared to QA-based columns: Multimodal properties of PrimaS® HR allow binding of AAV in neutral or closer to neutral pH values compared to QA-based columns.
• Reusability across multiple cycles reduces cost and supports high-throughput needs.
• Endotoxin Removal: Provides superior reduction of endotoxin compared to strong anion-exchangers (e.g. QA).
• cGMP-compliant: suitable for clinical and commercial production.

Your benefits:

• Very fast processes by convective mass transport – resolution independent of flow
• Ready to use columns
• High-capacity for large molecules due to fully accessible void volume in the channels
• Laminar flow minimizes shear stress, resulting in high yields