CIMmultus® r-Protein A-1mL 1.3µm

Tech Support

CIMmultus® r-Protein A columns are primarily intended for fast and efficient capture and purification of immunoglobulin G (IgG) and other r-Protein A-binding antibodies from various samples.

  • Primary binding to the hinge region of IgG-Fc of IgG, secondary binding to light chains of other antibody classes
  • Used for purification of mouse IgG, most subtypes of human IgG and many IgM monoclonal antibodies.

Notice: CIMmultus® r-Protein A columns are non standard products. Provided on request.

Item No.: 
311.1004-1.3
*Custom/bulk order quotes are provided within 72 hours of request.
CIMmultus® r-Protein A-1mL 1.3µm

CIMmultus® r-Protein A columns are primarily intended for fast and efficient capture and purification of immunoglobulin G (IgG) and other r-Protein A-binding antibodies from various samples.

  • Primary binding to the hinge region of IgG-Fc of IgG, secondary binding to light chains of other antibody classes
  • Used for purification of mouse IgG, most subtypes of human IgG and many IgM monoclonal antibodies.

Notice: CIMmultus® r-Protein A columns are non standard products. Provided on request.

Item No.:
311.1004-1.3

Overview

Specifications

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CIMmultus® Monoliths are reusable chromatography devices for scalable high-resolution purification of complex biological samples. Inside the custom designed housing is a single piece stationary phase with homogeneous channel size and surface chemistry. This stationary phase is designed to simplify method transfer from development into production.

They can be used as single-use disposable or multi-use columns, and they are available in various volume ranges – from 1mL for screening and method development purposes up to 40L for industrial manufacturing.

r-Protein A features:

  • Primary binding to the hinge region of IgG-Fc of IgG, secondary binding to light chains of other antibody classes
  • Used for purification of mouse IgG, most subtypes of human IgG and many IgM monoclonal antibodies.

Benefits

  • Very fast processes by convective mass transport – resolution independent of flow
  • Ready to use columns
  • High-capacity for large molecules due to fully accessible void volume in the channels
  • Laminar flow minimizes shear stress resulting in high yields

Applications

  • Application
    • Preparative protein purification
    • Preparative Applications
    • Protein and mAb production

Compliance Information

  • GMP Compliance
    non-cGMP

Connections (Physical)

  • Connector(s)
    10-32 UNF coned port, 0.0625" OD tubing connection

Dimensions

  • Channel Size
    1.3 µm
  • Column Tube Length
    4.2 mm
  • Inner Diameter
    6.7 mm
  • Outer Diameter
    18.6 mm

Features

  • Reusable
    Yes

General Specifications

  • Flow Rate
    0.1–16 mL/min | 4.2–625 cm/h | 0.1–16 CV/min

Materials of Construction

  • Materials of Construction
    Poly(butyl methacrylate -co- ethylene dimethacrylate)

Operating Conditions

  • Maximum Operating Pressure
    1.8 MPa | 18 bar | 260 psi

Physicochemical Information

  • Bed Volume
    1 mL
  • Ligand
    r-Protein A (affinity, recombinant protein A produced from E. Coli coupled to CDI-preactivated support)

Product Information

  • Brand
    CIMmultus®
  • Package Type
    Individually Packed

Volume

  • Volume
    1 mL