INTERFERin® siRNA/miRNA Transfection

Publications have shown that transfecting high siRNA concentrations can lead to non-specific off target effects. The reliability of the experimental data can be increased by using as little siRNA as possible. This is why INTERFERin^® has been specifically designed to provide high silencing efficiency using low siRNA concentrations. INTERFERin^®-mediated delivery of 1 nM of a specific siRNA shows selective and highly efficient knockdown of gene expression, while a competitor (L2K) needs at least 10 nM siRNA to reach 50% silencing efficiency.

Transfection of microRNA (miRNA) oligonucleotides is increasingly being used to analyze biological effects of specific miRNAs on cell function. INTERFERin^®is the reagent of choice for delivering miRNA, miRNA mimics or pre-miRNAs. INTERFERin^® is the latest generation siRNA & miRNA transfection reagent, especially designed for high transfection efficiency in a wide variety of cells, resulting in high gene silencing or stimulation of gene expression. Indeed, some miRNA are also known to induce gene expression by association with the promoter of the gene of interest. For example, INTERFERin^®-mediated delivery of miR-373 leads to a 3 fold increase in E-Cadherin expression.

For many adherent cell lines or primary cells, 1 nM siRNA is sufficient to obtain more than 90 % gene silencing. For suspension cell lines, 80 % silencing can still be reached by INTERFERin^® using 5 nM siRNA.

When it comes to cell viability, INTERFERin^® outperforms other transfection reagents. 48 h after transfection with 1 nM siRNA, cells transfected with INTERFERin^® appear healthy, while toxicity is clearly observed with reagent S (Fig. 3). 

When it comes to cell viability, INTERFERin^® outperforms other transfection reagents. 48 h after transfection with 1 nM siRNA, cells transfected with INTERFERin^® appear healthy, while toxicity is clearly observed with reagent S.