iQue® Human NK Cell Killing Kit

The Human General Immune Cell Mediated Killing Kit was designed for ease of use in multiplexing cellular phenotypic and functional markers and bead-based, secreted cytokine measurements in the same assay. This no wash assay requires minimal hands on time and measures both cells and beads to provide the following biological insights:

  • Live immune cells are distinguished from dead cells by staining with fluorescent membrane integrity dye.
  • Target cells are distinguished from effector cells by staining with a fluorescent encoder dye.
  • NK cells are immunophenotyped by staining with a fluorescent antibody panel to separate CD3- CD56+ NK cells.
  • The panel also includes 2 different NK cell functional activation markers, CD69 and CD25.
  • Optimized workflow enables the measurement of pro-inflammatory effector secreted cytokine IFNγ and pro-apoptotic serine protease Granzyme B in the same assay well.

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Key Benefits

Speed: Sample to actionable data allows multiple assays to be run in one experiment thereby reducing the hands on time, required numbers of overall tests and conserving precious sample.

Results: Nine endpoints from a single well assay measuring activation markers (CD69 and CD25), cell membrane integrity, cell count, secreted pro-inflammatory cytokine IFNγ, and pro-apoptotic protease Granzyme B.

Biological insights: Generate functional data and analyze cell health from same cell by multiplexing cell phenotypes and function in a single well.

Ease of use: Single platform assay and data analysis with ForeCyt® eliminates the time required for acquiring and correlating data from multiple sources.


  • Application
    Immune Cell Function  
  • Research Area
    • Immuno-Oncology  
    • Antibody Discovery  
    • Drug Discovery  

Biological Information

  • Target

General Specifications

  • Laser Configuration
  • Reagents and Kits Type
    NK Cell Killing  

Product Information

  • Brand
  • Pack Size
    1 X 96 wells  

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