jetMESSENGER® mRNA Transfection

Tech Support Documents
jetMESSENGER® mRNA Transfection
 

jetMESSENGER® is a novel powerful transfection reagent that has been specifically designed for high mRNA transfection efficiency in easy and hard-to-transfect cells such as primary cells, cancer cell lines, neurons, and stem cells. Transfection with jetMESSENGER® leads to a very low cytotoxicity as it requires low amounts of mRNA and low volumes of reagent.

Amount of Reagent
Price (USD):
$491.00
Item No.: 
101000005
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

Capabilities

Outperforms DNA transfection

jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. mRNA transfection presents many advantages versus DNA transfection:

  • No risk of genome integration, hence no genome modification of the transfected cell
  • No promoter regulation issue
  • No need to reach the nucleus for efficient expression
  • More gentle process

Allows higher gene expression than main competitors on a variety of cell lines

jetMESSENGER® is extremely efficient especially in hard-to-transfect cells compared to other mRNA transfection reagents. Transfection with jetMESSENGER® leads to outstanding mRNA delivery in a wide variety of cell lines, such as neurons, primary cells, stem cells and various cancer cell lines,

Suitable for different applications

jetMESSENGER® is designed for several applications, such as gene expression, CRISPR/Cas-9 gene editing, reprogramming experiments in fibroblasts and stem cells, and immunotherapy assays.

Extremely gentle on cells

By eliminating the need to reach the nucleus for efficient expression, jetMESSENGER® allows transfection of quiescent and slow dividing cells. In addition, jetMESSENGER® operates through an extremely gentle process. Cell viability remains extremely high during transfection and cell morphology is maintained.

jetMESSENGER® is a highly efficient and gentle mRNA transfection reagent. It has been specifically designed to offer outstanding transfection efficiency in cells that are usually difficult to transfect, such as primary cells, cancer cell lines, neurons, and stem cells. they can also be used on a wide variety of easy to transfect cells. Transfection with jetMESSENGER® leads to very low cytotoxicity as it requires low amounts of mRNA and low volumes of reagent.And it’s perfectly suited for gene expression, CRISPR gene editing, iPS generation, stem cell differentiation and immunotherapy assays.Key Benefits:

  • High efficiency transfection: Utmost gene expression on a wide variety of hard-to-transfect cells.
  • Keeps Cell Integrity: Extremely gentle on cells.
  • Cost Saving: Low amounts of mRNA and low volumes of reagent required.
  • Outperforms DNA Transfection: No need to reach the cell nucleus.

Applications

  • Application
    • Gene expression
    • Genome Editing
    • Stem cell differentiation
    • Reprogramming
    • Immunotherapy
  • Cell Type
    • Hard-to-transfect cells
    • Adherent and suspension cells
    • Primary cells (Neurons, Stem cells, Immune cells, fibroblasts, …)
    • Post-mitotic cells

Biological Information

  • Buffer Size
    60 mL
  • Molecule delivered
    mRNA
  • Number of transfections
    1.5 ml of jetMESSENGER® transfection reagent is sufficient to perform up to 1500 transfections in 24-well plates or 375 transfections in 6-well plates

General Specifications

  • Amount of Reagent
    jetMESSENGER® 0.75 mL
  • Reagents and Kits Type
    jetMESSENGER®

Product Information

  • Provided With
    mRNA buffer

Storage Conditions

  • Storage Conditions
    5 °C ± 3 °C