pHrodo® Zymosan Bioparticles® for Incucyte®
One vial of 1 mg sterile lyophilized solid sufficient for 100-200 live-cell imaging phagocytosis tests in 96-well format.
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pHrodo Bioparticles® for Incucyte® are sterile fluorogenic reagents ideally suited to a simple mix-and-read, real-time live cell quantification of phagocytosis. The unique pHrodo®-based system exploits the acidic environment of the phagosome to quantify phagocytosis. As pHrodo Bioparticles® for Incucyte® residing in the neutral extracellular solution (pH 7.4) are engulfed by phagocytes and enter the acidic phagosome (pH 4.5 – 5.5), a substantial increase in fluorescence is observed. Application of pHrodo Bioparticles® for Incucyte® to non-phagocytic cells yields little or no fluorescent signal. With the Incucyte® integrated analysis software background fluorescence is minimized. These fully sterilized reagents have been validated for use with the Incucyte® Live-Cell Analysis System and enable real-time evaluation of phagocytic regulation by pharmacological agents as well as genetic and environmental factors.
Research AreaCell Function
Species +/- IsotypeZymosan