PPA HyperCel is a mixed-mode resin with an aromatic ligand exhibiting stronger hydrophobicity and providing a different selectivity in protein purification. Pre-packed columns simplify resin screening and selection, and are ideal for process step optimization. The RoboColumn format is especially suited to high throughput process development (HTPD).

Bed Volume
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PPA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a phenlypropylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. PPA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC


  • Application
    Resin screening and process optimization  

Compliance Information

  • Quality Standards


  • Bead Size
    80 - 100 µm  
  • Bed Height
    50 mm  
  • Dimensions
    5 x 50 mm  

General Specifications

  • Flow Rate
  • Resin Format
    Pre-packed column  

Materials of Construction

  • Base Matrix
    Rigid cellulose beads  
  • Materials of Construction

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)  
  • pH Stability
    1 to 14 (short term), 2 to 12 (long term)  

Physicochemical Information

  • Bed Volume
    1 mL  
  • Ligand
  • Reference Protein
    Bovine serum albumin (BSA)  
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA  

Product Information

  • Brand
    PPA HyperCel  
  • Pack Size
    1 column per pack  
  • Pack Volume
    1 mL  
  • Resin Delivery Condition
    24% iPrOH/100mM sodium phosphate, pH 4.3  
  • Resin Type
    Mixed-mode anion exchanger  
  • What you receive
    1 column, instructions  

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl