PULSin® Protein Delivery
The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.
- Price (USD):
- $513.00
- Item No.:
- 101000010
- Availability:
- pcs
*Custom/bulk order quotes are provided within 72 hours of request.
Capabilities
PULSin® enables delivery of protein irrespective of its size
PULSin® is able to deliver R-phycoerythrin, a fluorescent protein (240 kD), to the cytoplasm of up to 98% cells. The protein is evenly distributed in the cytoplasm and excluded from the nucleus due to its large size.
PULSin® efficiently delivers antibodies in live cells
Antibodies are successfully delivered in mammalian cells and able to recognize their target protein inside the cytoplasm. PULSin® enabled the delivery of FITC-labeled anti-alpha-tubulin to the cytoplasm of high percentage of HeLa cells (85%). Similarly, anti-giantin Alexa Fluor® 488 was delivered to the cytoplasm of 98% of live HeLa cells, labeling the Golgi apparatus.
Simple protocol for successful protein delivery.
PULSin® will save you time and efforts compared to other techniques using viral transduction or chemical conjugation. PULSin® reagent is ready-to-use and provided with a dilution buffer and a fluorescent control protein (R-phycoerythrin).
The protocol is fast: simply add the reagent to the protein, incubate and add to the cells. Cells can be analyzed starting 4 hours post-delivery.
Easy to use alternative to chemical conjugation and viral transduction.
Comparison of PULSin® with two other protein delivery reagents shows a higher efficiency of protein delivery, Moreover, the amount of protein delivered per cell is higher with PULSin® as measured for R-phycoerythrin protein and for FITC-alpha-tubulin antibody (cf additional data).
Choose your Transfection Reagent For Life Science Research
PULSin® is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.Key Benefits:
- Unique: One reagent for protein, antibody, and peptide delivery into mammalian cells.
- Optimized: Efficient delivery in live primary cells and difficult to transfect cells.
- Innovative: Easy to use alternative to chemical conjugation and viral transduction
Applications
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Cell TypeAdherent & suspension cells grown in presence of serum
Biological Information
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Hepes dilution buffer20 mL
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Molecule deliveredProtein, Antibody, Peptide
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Number of transfections0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates
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Positive control (R-phycoerythrin)20 µg
General Specifications
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Amount of ReagentPULSin® 0.4 mL
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Reagents and Kits TypePULSin® - Protein delivery
Product Information
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Provided WithHepes Buffer (20mM) for protein dilution, R-Phycoerythrin used as a positive control
Storage Conditions
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Storage Conditions5 °C ± 3 °C