PULSin® Protein Delivery

The delivery of protein and antibody using PULSin® represents a powerful approach for functional studies. With PULSin® you can target intracellular proteins with antibodies in living cells without a fixation step, as well as study the function of a protein by controlling the expression level and the expression time course.

Amount of Reagent
Price (USD):
$513.00
Item No.: 
101000010
Availability: 

*Custom/bulk order quotes are provided within 72 hours of request.

PULSin® is composed of a proprietary cationic amphiphile molecule that forms non-covalent complexes with proteins and antibodies. Once formed, complexes are internalized via anionic cell-adhesion receptors and are released into the cytoplasm where they disassemble. The process is non-toxic and delivers functional proteins.Key Benefits:

  • Unique: One reagent for protein, antibody, and peptide delivery into mammalian cells.
  • Optimized: Efficient delivery in live primary cells and difficult to transfect cells.
  • Innovative: Easy to use alternative to chemical conjugation and viral transduction

Applications

  • Cell Type
    Adherent & suspension cells grown in presence of serum  

Biological Information

  • Hepes dilution buffer
    20 mL  
  • Molecule delivered
    Protein, Antibody, Peptide  
  • Number of transfections
    0.4 ml of PULSin® delivery reagent is sufficient to perform 25 delivery experiments in 6-well plates and 100 experiments in 24-well plates  
  • Positive control (R-phycoerythrin)
    20 µg  

General Specifications

  • Amount of Reagent
    PULSin® 0.4 mL  
  • Reagents and Kits Type
    PULSin® - Protein delivery  

Product Information

  • Provided With
    Hepes Buffer (20mM) for protein dilution, R-Phycoerythrin used as a positive control  

Storage Conditions

  • Storage Conditions
    5 °C ± 3 °C