Vivaflow® 50, 3,000 MWCO PES, 2pc
100 ml - 3 l
- Plug and play laboratory cross flow cassette
- Modular - scalable to 6 units
- Optimal for aqueous sample concentration e.g. proteins and nanoparticles
- High flow rate and recovery
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Vivaflow® 50 is a disposable and ready-to-use crossflow cassette for filtration and concentration of samples from 100 ml to 3 l. The membrane surface per device is 50 cm2. One pack contains two devices and comes with all necessary accessories for running two devices with a laboratory pump and a size 16 pump head. For speeding up the concentration and increasing the volume throughput, six cassettes can be connected.
Optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration e.g. in fish breeding facilities, this economical product offers a standard of ease of use, reliability and flexibility unrivalled by any laboratory filtration system in the market. The thin-channel, flip-flow recirculation path of the modules provides high crossflow velocities with minimum pump speed requirements. A single 50 cm2 module typically reduces 500 ml to 15 ml in less than 50 min. Nearly total recovery of the concentrate is achieved with a single rinse. Up to six of the unique interlocking modules can be connected for easy scale up resulting in a capacity of several liters. Vivaflow® 50 is intended for single use. All accessories for sample concentration, except a peristaltic pump with a pump head for size 16 tubing, are included in the package. A 500 ml sealed diafiltration reservoir can be added to the system for rapid and convenient buffer exchange.
The standard Vivaflow® 50 package comes with two cassettes, tubing, flow restrictor, one series interconnector and high pressure pump tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD tubing (size 16).
Running more than two Vivaflow® 50 devices in parallel and series
For speeding up the concentration, as many as six Vivaflow® 50 cassettes can easily be connected using 2 T-connectors for connecting 2 Vivaflow® cassettes at the inlet and at the outlet and series interconnectors for connecting the devices in between. A pressure indicator needs to be built in the inlet for optimal control.
- High flow rate and recovery
- Clear housing for process monitoring
- Ready to use single cassettes
- Easy set up of multiple cassettes
- Economical and disposable product
- Unique in its performance
A single 50cm2 module will typically reduce 500 ml to less than 15 ml in under 50 minutes. Less than 10 ml minimum system recirculation for highest concentrations. Less than 1.5 ml non recoverable hold up volume. Near total recoveries achievable with a single 10 ml rinse.
Application AreaBuffer Exchange
Electrical / Battery itemNo
Channel Height0.3 mm
Channel Width15 mm
Membrane Area50 cm²
Overall Height84 mm
Overall Length107 mm
Pump Flow200 – 400 mL/min
TubingPVC (medical grade)
Materials of Construction
Flow ChannelTPX (PMP)
Flow RestrictorPolypropylene (PP)
Housing MaterialPolycarbonate (PC)
Material o-ring | sealSilicone (SI)
Membrane MaterialPolyethersulfone (PES)
Membrane SupportTPX (PMP)
Pressure IndicatorPolypropylene (PP), Stainless Steel (SS), Polyoxymethylene (POM), Silicone (SIL), Polyamide (PA)
Maximum Allowable Working Temperature60 °C (140 °F)
Maximum Operating Pressure3 bar (44 psi)
Non-recoverable Hold-up< 0.5 mL
Device TypeTFF – (Crossflow)
Dead Stop VolumeN/A
Hold-up Volume1.5 mL
Minimum Recirculation Volume10 mL
Volume Range100 – 3,000 mL
Tubing Set for Vivaflow® 50
Comprises: 2 x 1 m size 16 PVC tubing with inlet fittings, 2 x 50 cm size 16 PVC tubing with 0.6 mm flow restrictors, 1 x series interconnector
T Connectors for Vivaflow® 50 (2 pieces)
T connectors for running 2 stacks of Vivaflow® 50
Plate used for setting up Vivaflow® 50 units
Vivaflow® 50 stand
Series Interconnectors (6 pieces)
Series interconnectors for running up to six Vivaflow® 50 units in parallel
Pressure Indicator (1-3 bar)
Pressure indicator (1-3 bar) for Vivaflow® units
Pumps used for filtration. Choose from a broad range of universal pumps and manual pumps over laboratory vacuum pumps to peristaltic pumps.
500 ml sample|diafiltration reservoir to be used with Vivaflow® systems. Allows the convenient application of samples and the continuous replacement of buffer.
Masterflex Easy Load Pump Head - Size 16
Flow restrictor 0.6 mm (6 pieces)
Flow restrictors 6 x 0.6 pink to be used with the Vivaflow® systems
Female Luer fittings Size 16 (10 pieces) to be used with the Vivaflow® systems