Vivaflow® TFF Cassette, PES

Vivaflow® 200 is a ready to use and reusable laboratory crossflow cassette for concentrating up to 2.5L samples. The active membrane surface area per device is 200 cm2. One unit is supplied with all necessary tubing, fittings and accessories for running the device with a laboratory pump and a size 16 pump head. For faster concentration or to process up to 5 L samples, two cassettes can be operated simultaneously, in parallel.

Top Features of Vivaflow® PES

  • Ready-to-use, reusable
  • Rapid sample processing
  • Two cassettes can be operated in parallel
  • Near-total recoveries with a single buffer rinse
  • Conveniently supplied with tubing, pressure indicator, flow restrictor and tubing

Popular Applications for Vivaflow® PES

  • Cell culture supernatant concentration
  • Biopharmaceutical research
  • Virus and VLP concentration

Price (USD):
Item No.: 

*Custom/bulk order quotes are provided within 24 hours of request.

Vivaflow® multi-use laboratory crossflow cassettes are versatile, economical options for concentration and diafiltration in various applications. The cassettes are ready to use out of the box, and may be re-used to process several samples. They are simple and efficient, utilizing a unique flip-flow channel design that ensures optimal flux with no optimization effort. These cassettes are easy to use, reliable, and the best choice given their unrivaled flexibility compared to any laboratory ultrafiltration system in the market.

Cassette Equipment

Each Vivaflow® package conveniently includes tubing, pressure indicator, flow restrictor and tubing. All you need is a peristaltic pump with a pump head capable of handling 6.4 mm OD (size 16) tubing.

Rapid Sample Processing

Vivaflow® cassettes offer unmatched speed and efficiency. A single 200 cm2 device typically concentrates 1L by a factor of 50 in less than 30 min. Alternatively, two Vivaflow® 200 cassettes can be used in parallel for the concentration of 5L in under 75 minutes. Near-total recovery of the concentrate is achieved with a single buffer rinse.

Parallel Operation

For even faster process speeds, two Vivaflow® 200 cassettes can be operated simultaneously. Easily connect two cassettes using a Y connector, pre-assembled with the correct tubing sizes and fittings. This modular operation enables samples up to 5L to be concentrated.

Cassettes Can be Used in Multiple Applications

Vivaflow® 200 is optimally suited for applications like cell culture supernatant concentration, virus concentration and water concentration (e.g. in fish breeding facilities). In biopharmaceutical research, the cassettes can be used for recombinant protein (e.g. mAb) concentration and diafiltration. They can also be used for nanoparticle research processes, including concentration, diafiltration or low molecular weight contaminant removal. Utilize them for the concentration of environmental samples before analysis of trace metals or pathogens and much more.


  • Application
    • Macromolecule Concentration
    • Desalting
    • Buffer Exchange
  • Industry
    Pharma / Biotech
  • Intended Use
    Research Use Only (RUO)
  • Market
    • Research
    • Environmental

Compliance Information

  • Certification
    ISO 9001

Connections (Physical)

  • Fittings
    Polyamide (PA)


  • Channel Height
    0.4 mm
  • Channel Width
    10 mm
  • Dimensions WxDxH
    126 x 38 x 138 mm
  • Membrane Area
    200 cm²
  • Overall Height
    138 mm

General Specifications

  • Pump Flow
    200 – 400 mL/min
  • Tubing
    PVC (medical grade)

Materials of Construction

  • Flow Channel
  • Flow Restrictor
    Polypropylene (PP)
  • Housing Material
  • Material o-ring | seal
    Silicone (SI)
  • Membrane Material
    Polyethersulfone (PES)
  • Membrane Support
    Polypropylene (PP)
  • Pressure Indicator
    Polypropylene (PP), Stainless Steel (SS), Polyoxymethylene (POM), Silicone (SIL), Polyamide (PA)

Operating Conditions

  • Maximum Allowable Working Temperature
    60 °C (140 °F)
  • Maximum Operating Pressure
    4 bar (58 psi)

Physicochemical Information

  • MWCO
    10 kDa
  • Non-recoverable Hold-up
    2 mL

Product Information

  • Brand
  • Device Type
    TFF – (Crossflow)
  • Pack Size
  • Sample Type
    • Protein
    • Peptide
    • Nanoparticles
    • Virus
    • Nucleic Acid
    • VLP
    • Exosome


  • Dead Stop Volume
  • Hold-up Volume
    5.3 mL
  • Minimum Recirculation Volume
    20 mL
  • Volume Range
    500 – 5,000 mL
  • Replacement Tubing Set for Vivaflow®


    Tubing Set for Vivaflow® 50

    Comprises: 2 x 1 m size 16 PVC tubing with inlet fittings, 2 x 50 cm size 16 PVC tubing with 0.6 mm flow restrictors, 1 x series interconnector

  • Pressure Indicator, 1-3 bar


    Pressure Indicator (1-3 bar)

    Pressure indicator (1-3 bar) for Vivaflow® units

  • Flow Restrictors, 0.6 mm, 6pc
    Item no.
    VFA035 Discontinued

    Flow restrictor 0.6 mm (6 pieces)

    Flow restrictors 6 x 0.6 pink to be used with the Vivaflow® systems

  • Luer Fittings, Female to Size 16 Hose Barb, 10pc


    Female Luer fittings Size 16 (10 pieces) to be used with the Vivaflow® systems

  • Size 16 Pump Tubing, PVC, 3 m


    • Size 16 pump tubing for Vivaflow®
    • Size 16 PVC pump tubing (3 m, 3.2 x 1.6 mm) and Luer fittings

  • Luer Fittings, Female to Size 15 Hose Barb, 10pc


    Female luer fittings size 15 (10 pieces)

    Female Luer fittings Size 15 to be used with the Vivaflow® systems

  • Peristaltic Load Pump Head, Size 16


    Masterflex Easy Load Pump Head - Size 16

  • Size 15 Pump Tubing, PVC, 3 m


    Tygon Size 15 pump tubing for Vivaflow® 200

    Size 15 pvc pump tubing (3 m,4.8 x 2.6 mm) and Luer fittings

  • Peristaltic Pump Head, Size 15


    Masterflex Easy Load Pump Head - Size 15

  • Flow restrictors, 0.4, 0.6 and 0.8 mm (2 of each)


    Flow restrictor set (2 x 0.4, 0.6, 0.8 mm)

    Flow restrictor set (2 x 0.4, 0.6, 0.8 mm) to be used with Vivaflow® systems

  • Peristaltic Pump Drive, 115v


    Pumps used for filtration. Choose from a broad range of universal pumps and manual pumps over laboratory vacuum pumps to peristaltic pumps.

  • Y-Connector,  Size 15 to 2x Size 16


    Y connector (size 15 to 2 x size 16) for operating two Vivaflow® 200 units

    Y Connector (size 15 to 2x size 16)

  • Sample and Diafiltration Reservoir, 500 mL


    500 ml sample|diafiltration reservoir to be used with Vivaflow® systems. Allows the convenient application of samples and the continuous replacement of buffer.

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