HA Ultrogel®

Tech Support Documents
HA Ultrogel®
 

HA Ultrogel® is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. A wide variety of applications include separation of proteins, peptides and nucleic acids, from laboratory to production scale.

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HA Ultrogel®
HA Ultrogel®

HA Ultrogel® is a composite material of cross-linked agarose and microcrystalline hydroxyapatite enclosed in the agarose matrix. The material shows mixed mode functionality based on cation exchange and metal affinity in the hydroxyapatite structure. A wide variety of applications include separation of proteins, peptides and nucleic acids, from laboratory to production scale.

Item No.:
M_HA_Ultrogel

Overview

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HA Ultrogel® resin is a hydroxyapatite agarose composite resin for the separation of biomolecules from research and to manufacturing scale. Hydroxyapatite chromatography is considered to be a “pseudo-affinity” chromatography, or “mixed mode” ion exchange. It has proven to be an effective purification mechanism in a variety of processes, providing biomolecule selectivity complementary to more traditional ion exchange or hydrophobic interaction techniques.

Features and Benefits

  • Unique selectivity and separation mechanism, complementary to traditional IEX and HIC
  • Protein purification in neutral, non-denaturating conditions
  • High porosity, easy cleaning

Applications

  • Purification of human serum proteins
  • Purification of basic proteins, of phosphate-dependent proteins and enzymes, and of DNA-dependent enzymes
  • Vaccine purification processes, e.g. Bordetella pertussis toxin
  • Separation of plant proteins such as lectins, glycoproteins, glycosidases, phospholipidases, sulfohydrolases, sphingomyelinases, transferases, trehalases and kinases

Applications

  • Application
    Preparative protein purification

Compliance Information

  • Quality Standards
    • CoA
    • RSF (upon request)

Dimensions

  • Bead Size
    60-80 µm

General Specifications

  • Flow Rate
    < 100 cm/h
  • Resin Format
    Bulk resin

Materials of Construction

  • Base Matrix
    Agarose

Operating Conditions

  • Operating Pressure
    < 2 barg (44psi)

Physicochemical Information

  • Ligand
    Hydroxyapatite
  • Reference Protein
    Cytochrome C
  • Typical Dynamic Binding Capacity 10% per mL
    > 7 mg/mL Cytochrome C

Product Information

  • Brand
    HA Ultrogel®
  • Resin Delivery Condition
    1M NaCl with 20% EtOH
  • Resin Type
    Mixed Mode cation exchanger
  • What you receive
    • 1 bottle
    • Instruction for use

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl