PPA HyperCel

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PPA HyperCel PPA HyperCel PPA HyperCel
 

PPA HyperCel is a mixed-mode resin with an aromatic ligand exhibiting stronger hydrophobicity and providing a different selectivity in protein purification.

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PPA HyperCel resin is an industry-scalable chromatography resin designed for protein capture and impurity removal in a biopharmaceutical environment. Operating on a “mixed-mode” mechanism, with a phenlypropylamine ligand, its behavior is based on a combination of electrostatic and hydrophobic interactions of the proteins with the ligands. PPA HyperCel resin provides unique selectivities, different from those given by ion exchange or conventional HIC (hydrophobic interaction chromatography).

Features and Benefits

- High mechanical and chemical stability
- Suitable for large-scale chromatography in a biopharmaceutical environment
- Cross-linked cellulose base matrix


Applications

- Protein purification at low ionic strength by direct hydrophobic capture
- Separation of challenging mixtures, e.g. protein isoforms or proteins with very close pIs
- Orthogonal separation mechanism to ion exchange or HIC

Compliance Information

  • Quality Standards
    • CoA
    • RSF

Dimensions

  • Bead Size
    80 - 100 µm

Materials of Construction

  • Base Matrix
    Rigid cellulose beads

Operating Conditions

  • Operating Pressure
    < 3 barg (44psi)
  • pH Stability
    1 to 14 (short term), 2 to 12 (long term)

Physicochemical Information

  • Ligand
    Phenylpropylamine
  • Reference Protein
    Bovine serum albumin (BSA)
  • Typical Dynamic Binding Capacity 10% per mL
    40-60 mg/mL BSA

Product Information

  • Brand
    PPA HyperCel
  • Resin Type
    Mixed-mode anion exchanger

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl