HEPES Buffer 1M

HEPES is a zwitterionic organic buffer that is also used to maintain physiological pH of cell culture media. HEPES is recommended when the cell culture system is very sensitive, increasing the buffering capacity and stabilizing the pH within the range of 7.2 to 7.6, and is not dependent on CO₂ levels.

Top Features

Zwitterionic buffer
Liquid formulation with high solubility
Easy-to-use 1M solutionIdeal for biochemistry, cell culture, and molecular biology applications
High solubility
Membrane impermeability
Limited effect on biochemical reactions
Very low visible and ultraviolet light absorbance
Chemically and enzymatically stable
Easy to prepare
pKa of HEPES is temperature dependent:

At 25ºC: pKa= 7.48

At 37ºC: pKa= 7.31

Volume

Price (USD):: $18.90
Item No.:: 03-025-1C
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Overview

HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. HEPES has no nutritional benefit to cells. It is added to the media solely for extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO₂ incubator.

Specifications

Applications

Application Area

Cell culture

Compliance Information

Animal Origin (AO) / Non-Animal Origin (NAO)

NAO
Quality Standards

ISO 9001 and 13485 Quality Management Systems (QMS) and in compliance with applicable cGMP guidelines