HEPES Buffer 1M

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HEPES Buffer 1M
 

HEPES is a zwitterionic organic buffer that is also used to maintain physiological pH of cell culture media. HEPES is recommended when the cell culture system is very sensitive, increasing the buffering capacity and stabilizing the pH within the range of 7.2 to 7.6, and is not dependent on CO2 levels.

Top Features

  • Zwitterionic buffer

  • Liquid formulation with high solubility

  • Easy-to-use 1M solutionIdeal for biochemistry, cell culture, and molecular biology applications

  • High solubility

  • Membrane impermeability

  • Limited effect on biochemical reactions

  • Very low visible and ultraviolet light absorbance

  • Chemically and enzymatically stable

  • Easy to prepare

  • pKa of HEPES is temperature dependent:

At 25ºC: pKa= 7.48

At 37ºC: pKa= 7.31

Volume
Item No.: 
03-025-1B
Availability: 

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HEPES is a zwitterionic organic buffer that is also used to maintain physiological pH of cell culture media. HEPES is recommended when the cell culture system is very sensitive, increasing the buffering capacity and stabilizing the pH within the range of 7.2 to 7.6, and is not dependent on CO2 levels.

HEPES is widely used in many biochemical reactions and as a buffering agent in some cell culture media. HEPES has no nutritional benefit to cells. It is added to the media solely for extra buffering capacity when cell culture requires extended periods of manipulation outside of a CO2 incubator.

Applications

  • Application Area
    Cell culture  

Compliance Information

  • Animal Origin (AO) / Non-Animal Origin (NAO)
    NAO  
  • Quality Standards
    ISO 9001 and 13485 Quality Management Systems (QMS) and in compliance with applicable cGMP guidelines  

Product Information

  • Pack Size
    1  
  • Package Type
    Bottle  

Sterility/Purity

  • Delivery Condition
    Sterile  

Storage Conditions

  • Storage Conditions
    Ambient Temperature  

Volume

  • Volume
    100 mL  

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