Blue Trisacryl® M - 100 mL
Blue Trisacryl® M is an affinity resin that is used for the purification of enzymes, interferons, and some coagulation factors, as well as for the removal of albumin. The resin uses a Trisacryl base matrix which carries the blue dye ligand.
- Item No.:
*Custom/bulk order quotes are provided within 72 hours of request.
Blue Trisacryl® M dye-affinity chromatography resin is used for the purification of a wide variety of enzymes and proteins such as kinases, interferons, and some coagulation factors. It is also used for albumin purification, or albumin depletion in samples for proteomics research.
Blue Trisacryl M resin is based on Trisacryl, a macroporous non ionic synthetic polymer on which blue dye is covalently immobilized. The blue dye interacts biospecifically with some proteins due to its structural similarity with nucleotide cofactors (ADP, NADP). Other proteins like albumin or interferon bind by a combination of hydrophobic, electrostatic and pi-pi interactions.
Features and Benefits
- Unique multi-modal interaction mechanism
- Low ligand leakage
- Multiple applications from laboratory to production scale
- Enzymes which need NAD as cofactor (kinases, dehydrogenases, phosphatases)
- Other enzymes (sulfatases, RNA polymerases, mono-oxygenases, oxidoreductases)
- Albumin from plasma
- Transferin from plasma
- α1-acid glycoprotein, α-fetoprotein, α1-proteinase inhibitor
- Serine proteases
- DNA antibodies
- Recombinant vaccines purification processes
ApplicationPreparative protein purification
Bead Size60 µm
Flow Rate100-200 cm/h
Resin FormatBulk resin
Materials of Construction
Base MatrixSynthetic Polymer
Operating Pressure< 3 barg (44psi)
pH Stability1 to 12 (short term), 4 to 10 (long term)
Typical Dynamic Binding Capacity 10% per mL>10 mg/mL HSA
BrandBlue Trisacryl® M
Pack Volume100 mL
Resin Delivery Condition1M NaCl with 20% EtOH
Resin TypeAffinity resin
What you receive1 bottle resin, instructions
Storage Conditions20% EtOH/1 M NaCl