Methyl Ceramic HyperD® F is a high-capacity sorbent/resin for Mixed-mode interaction (AEX and HIC) chromatography. It is specifically designed for the efficient and scalable purification of biomolecules. It provides superior dynamic capacity for proteins over a full range of linear velocities.

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Methyl Ceramic HyperD® F is designed for efficient and scalable capture of biomolecules

The sorbent:

  • Has a unique 'gel-in-a-shell' design that provides rapid mass transfer and effective binding
  • Employs a soft, high-capacity hydrophobic (-CH3) functionalized hydrogel which is polymerized within the large pores of a high dimensional rigid ceramic bead
  • Can be used for very hydrophobic target molecules or impurities
  • Does not shrink or swell with changes in pH or conductivity
  • Shows superior dynamic capacity for proteins over a full range of linear velocities

Features and Benefits

  • High dynamic binding capacity at high flow rates
  • Truly rigid, non-compressible resin

Applications

  • Efficient and scalable capture of biomolecules

Applications

  • Application
    Preparative protein purification  

Compliance Information

  • Quality Standards
    CoARSF upon request  

Dimensions

  • Bead Size
    50 µm  

General Specifications

  • Flow Rate
    >300cm/h with <3 barg  
  • Resin Format
    Bulk resin  

Materials of Construction

  • Base Matrix
    Ceramic Beads  

Operating Conditions

  • Operating Pressure
    < 3 barg (44 psi)  

Physicochemical Information

  • Ligand
    Methyl  
  • Ligand Density
    >250 µeq/mL  
  • Reference Protein
    • Bovine serum albumin (BSA)  
    • Human polyclonal IgG  
  • Typical Dynamic Binding Capacity 10% per mL
    > 50 mg/mL BSA at pH 4.5, 15 mS/cm; 60-100 mg/mL IgG at pH 4 to 5, 4-12 mS/cm  

Product Information

  • Brand
    Methyl Ceramic HyperD® F  
  • Pack Size
    1  
  • Resin Delivery Condition
    1M NaCl with 20% EtOH  
  • Resin Type
    Mixed-mode anion exchanger  
  • What you receive
    1 bottle resin, instructions  

Storage Conditions

  • Storage Conditions
    20% EtOH/1M NaCl