Sartocon® Cassette | Hydrosart® | 300kDa | 0.6m²

  • Membrane Material: Hydrosart®
  • Effective Filtration Area: 0.6m²
  • Cut-Off: 300 kDa
  • Screen Type: E-Screen (High viscosity)

Sartocon® TFF cassettes with Hydrosart® high performance membrane have been optimized for biopharmaceutical process applications.

Price (USD):
Item No.: 

*Custom/bulk order quotes are provided within 72 hours of request.

Sartocon Hydrosart® cassettes are characterized by ultra-low protein adsorption, easy cleaning, higher yields, and long product life. Even with repeated use, Hydrosart® ultrafiltration membrane maintains it’s performance without fouling or loss of retention.

Sartocon® cassettes are self-sealing, feature low extractables and the enlarged feed and retentate ports lower system pressure drops.

Sartocon® cassettes are ideally combined with Sartorius Sartocon holders and systems.


Typical 300 kDa TFF Applications are Ultrafiltration and Diafiltration of

  • Large Viruses such as MVA for Smallpox and Monkeypox vaccine
  • Virus like particles
  • pDNA and mRNA
  • Blood and Plasma
  • Protein solutions
  • Polysaccharide solutions
  • Large mAb constructs
  • Removal of Contaminants

Hydrosart® Membrane

Hydrosart® high performance membrane is a stabilized cellulose-based membrane that has been optimized for biopharmaceutical process applications. Hydrosart® is stable across a broad pH range, is extremely hydrophilic, making it non-protein binding, and virtually non-fouling. Membrane depyrogenation can be accomplished by using NaOH even at elevated temperatures. These features make Hydrosart® an ideal membrane for biological applications.

Flow Channel Geometry

The Sartocon® family is available in two flow channel geometry versions. E-Screen filters are dominantly used for protein concentrations up to above 20% as well as viscosity conditions greater than 3 cp. ECO-Screen devices mainly used for water based protein solutions of below 20% protein concentration as well as lower than 3 cp viscosity allow economical optimized filtration runs.

Available Sizes and Cut-Offs

Sartorius Sartocon® and Sartocube® TFF cassettes are available for pilot- and production scale, and in Sartocon® Slice as well as Sartocon® Slice 200 format for reduced volume handling.


E-screen: 2 | 5 | 10 | 30 | 100 | 300 kDa

ECO screen: 10 | 30 | 100 | 300 kDa

Regulatory Compliance

All product contact materials have passed the current USP Biological Test and Cytotoxicity Test. All materials are BSE and TSE free. The filtrate meets or exceeds the currently valid USP and EP for sterile Water for Injection, with respect to extractable substances, oxidizable substances and Bacteria Endotoxins.


  • Industry
    Pharma / Biotech  

Compliance Information

  • Bacterial Endotoxins
    < 0.18 EU/mL as determined by the LAL test  
  • Biological Reactivity
    All materials of this filter element meet the requirements of the current USP Biological Reactivity tests <88> for plastics Class VI (Systemic Injection, Intracutaneous and Implantation tests).  
  • Extractables
    The total amount of extractables is well below the limits established by the current USP unter "Sterile Water for Injection".  


  • Filtration Area
    0.6 m²  
  • Flow Channel Geometry
    Protein conc. >20% /or viscosity above.3cp  

General Specifications

  • Cassette Format
    Sartocon Cassettes  
  • Filter Class
  • Filter Format

Materials of Construction

  • Integral Frame
    Polyvinylidene fluoride (PVDF)  
  • Membrane Material
  • Sealing Compound
    Silicone White (High chemical resistance)  
  • Spacer
    Polypropylene (PP)  

Operating Conditions

  • Maximum Inlet Pressure
    4 bar  
  • pH Stability
    pH 2 – 14  

Physicochemical Information

  • MWCO
    300 kDa  
  • Non-Fiber Releasing
    This filter product complies with the title 21 of the Code of Federal Regulations (CFR), section 210.3(b)(6) and 211.72.  

Product Information

  • Brand
  • Pack Size
  • Type
    0.6m² | E-Screen  
  • Sartoflow® 20, 1.4435, F/R38/P26, DIN

    The Sartoflow® 20 stainless steel holder with hydraulic is designed for TFF applications from 200 L to 2500 L volumes. The holder is optimized for use with 4 Sartocon® up to 20 Sartocube® production scale TFF cassettes (max. 14 m2). Typical applications of the Sartoflow® 20 system includes concentration and diafiltration of proteins, vaccines, antibodies, oligo nucleotides, endotoxin removal etc., cell harvesting and clarification processes.