Spent Media Analysis

We have established validated methods for quantitation of various analytes, which we also make available to our customers. Our spent media analysis service covers for instance:

  • Amino acids (proteogenic, non-proteogenic)
  • Glucose and lactate
  • Trace Elements (e.g. Selenium, Iron)
  • Water-soluble vitamins
  • Organic acids
  • Dipeptides
  • Polyamines (Spermidine, Spermine, Putrescine)

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*Custom/bulk order quotes are provided within 72 hours of request.

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Products (20)

  • Phosphate Analysis by Photometric assay
    Item no.
    SMA-156
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Water-soluble Vitamins by UHPLC-MS/MS
    Item no.
    SMA-132
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • High Abundant Cation Analysis by ICP-MS
    Item no.
    SMA-186
    Sample Volume
    1 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Ascorbate (Vitamin C) Analysis
    Item no.
    SMA-135
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Glucose and Lactate Analysis
    Item no.
    SMA-162
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Analysis of Total Amino Acid Content
    Item no.
    SMA-115
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Glutathione (oxidized & reduced) Test
    Item no.
    SMA-149
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Amino Acid Analysis
    Item no.
    SMA-103
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Ammonium (Concentration) Analysis
    Item no.
    SMA-164
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Spent Media - Trace Element Bundle
    Item no.
    SMA-300
    Sample Volume
    1 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Glucose Analysis
    Item no.
    SMA-160
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Culture Media Analysis Bundle
    Item no.
    SMA-180
    Sample Volume
    2.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Glucose and Sucrose Analysis
    Item no.
    SMA-137
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Peptide Size Determination by SEC
    Item no.
    SMA-220
    Sample Volume
    0.5 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
  • Element Analysis Bundle (5 elements)
    Item no.
    SMA-308
    Sample Volume
    1 mL
    Application
    Cell Culture Media, Spent Media, Feed Supplements
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    Cell culture involves growth of cells in an artificial environment. Cell culture medium is a liquid created to support the growth of cells in this environment. Usually, it contains a mixture of:an energy source like carbohydrates in the form of sugarsamino acids since they are essential building blocks of proteinsfatty acids and lipids are particularly important in serum-free mediainorganic salts to maintain osmotic balancevitamins and trace elements essential for growth and proliferation of cellsSpent media is the 'waste' media that remains after cell cultivation step. Nutrient depletion and metabolite accumulation occur in the medium during cell growth. Spent media analysis is the study of this waste media over the course of the biomanufacturing step to provide insight on this process. We have established validated methods for quantitation of various analytes, which we also make available to our customers. Our spent media analysis service covers for instance:Amino acids (proteogenic, non-proteogenic forms; all 20 amino acids and additional analytes)Trace Elements (30+ elements including manganese, selenium, iron, copper, etc)Water soluble vitamins (Choline, Folate, Panthothenate, Biotin, Thiamine, etc.)Organic acids (e.g., citric acid, fumaric acid, lactic acid, malic acid, pyruvic acid, succinic acid, and tartaric acid)Dipeptides (e.g., Alanyl-glutamine, Glycyl-tyrosine)Polyamines (i.e. Spermidine, Spermine, Putrescine)Inorganic anions (e.g., Chloride, Sulfate, Phosphate, etc.)Inorganic cations (e.g., Sodium, Potassium, Calcium, Magnesium, etc.)Glucose, sucrose and lactateAmmoniumAscorbate (Vitamin C)PurineSample Requirements: Samples can be spent cell culture media or cell culture supernatants. Please read and sign our sample declaration sheet. Prepare and ship your samples according to our sample preparation protocol.Deliverables: We will send you a short report summarizing your data within 5 business days (After receipt of samples and if no additional sample preparation / matrix evaluation is necessary. Valid for sets of more than 5-50 samples).