4Cell® XtraCHO Media and Feeds
4Cell® XtraCHO is a proprietary media formulation developed by Sartorius for the growth and maintenance of the Sartorius (Cellca) CHO-DG44 cell from cell line development projects delivered until the end of 2022. It provides robust performance in small and large scale production systems such as flasks, Ambr® 15 | 250 and bioreactors.
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4Cell® XtraCHO is a chemically defined, protein-free, serum-free, and hydrolysate-free media. The portfolio includes 4 different formulations:
- Stock & Adaptation Media (SAM) allow the cultivation or media adaptation of cells.
- Production Media (PM) are a non-selective production media for batch or fed-batch cultures. It is used for the effective cultivation of CHO DG44 cells and helps produce high yields of the active protein.
- Feed Medium A (FMA) and Feed Medium B (FMB) are feed additives especially matched to the production medium. Combining those increases fed-batch productivity 3- to 5-fold compared to batch culture conditions.
4Cell® XtraCHO Media are formulated to maximize product titers of the Sartorius CHO-DG44 cell lines (from cell line development projects delivered through the end of 2022). It provides robust performance in small- and large-scale production systems such as flasks, Ambr® 15 | 250 and bioreactors.Available in liquid (bottle & Flexsafe® bags) and powder formats.
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