Incucyte® Cytotox Dye for Counting Dead Cells

Supplied as solution in dimethyl sulfoxide (DMSO), providing sufficient quantity for performing 500–1000 tests (1 test = 1 well of 96-well microtiter plate).

Top Features of Incucyte® Cytotox Dye

  • Detect cytotoxicity in real time with non-perturbing reagents
  • Reduce loss of compromised cells with simple, mix-and-read protocols
  • Acquire reliable data with little or no intrinsic fluorescence
  • Multiplex with proliferation and apoptosis measurements

Popular Applications for Incucyte® Cytotox Dye

  • Ideal for detection of cell membrane integrity disruption and quantitation of cell death in real-time
  • Multiplexed applications, such as apoptosis, proliferation, and single spheroid growth, when combined with other Incucyte® reagents and metrics
  • NETosis

Note: This product is not returnable. If you have any issues with this product, please contact your Sartorius sales representative for assistance.

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Incucyte® Cytotox Dyes are formulated to detect cell membraned integrity disruption in real time to quantify cell death. These dyes are ideally suited to the mix-and-read, real-time quantification of cells undergoing cytotoxicity. When combined with Incucyte® Live-Cell Analysis Systems and integrated software, these reagents allow for reliable, accurate evaluation of cytotoxicity. These dyes can also be used in conjunction with other Incucyte® reagents for multiplexed readouts of cytotoxicity, proliferation and apoptosis within the same well. Addition of the Incucyte® Cytotox Dyes to normal healthy cells is non-perturbing to cell growth or morphology and yields little or no intrinsic fluorescent signal.

Once cells become unhealthy, the plasma membrane integrity diminishes, allowing entry of the Incucyte® Cytotox Dye and yielding a 100- to 1000-fold increase in fluorescence upon binding to deoxyribonucleic acid (DNA). With the Incucyte® integrated analysis software, fluorescent objects can be quantified, and background fluorescence minimized.

Presentation, Storage and Stability

Incucyte® Cytotox Dyes are supplied as a solution in dimethyl sulfoxide (DMSO), providing sufficient quantity for performing 500–1000 tests (1 test = 1 well of 96-well microtiter plate). Upon receipt, the solution should be stored at -20°C or 4°C, depending on the reagent, and protected from light. When stored as described, the stock solutions will be stable for 6 - 12 months.

Recommended Use

We recommend that each vial of Incucyte® Cytotox Green or Red Dye is diluted to a stock concentration of 100 µM in full media or PBS. This may then be diluted further in full media for direct addition to cells seeded in a 96-well plate to yield a final concentration of 250 nM (e.g., 1:4000 dilution of the original green or red dye).

Incucyte® Cytotox NIR Dye should be diluted to a final concentration of 0.6 µM (1:000 dilution) in full culture media for use.

When used in an Incucyte® Live-Cell Analysis System, we recommend data collection every 2-3 hours. Additional protocol details can be found in the Product Guide.


  • Application
    • Cytotoxicity  
    • Spheroid Cell Health  
  • Assay Type
    • Cytotoxicity  
    • NETosis  
  • Research Area
    Cell Health  


  • Device/Instrument Compatibility
    • SX1  
    • S3  
    • SX5  
  • Optical Module

General Specifications

  • Color

Product Information

  • Brand
  • Pack Size

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