BioPAT® Trace

BioPAT^® Trace analysis platform is designed for simultaneous online monitoring of up to two parameters in any given setup. The biosensor components measure either glucose and lactate or small-molecule alcohols, such as methanol and ethanol.

The system can be used for laboratory or industrial cultivations of microorganisms and mammalian cell lines.

BioPAT^® Multi Trace has all the measurement functionality of the dedicated system yet is capable of sampling from up to four vessels without the need for operator intervention. The frequency, relative sample volume and accuracy of the parameter concentration data allows automated parameter control. This can be achieved by either actuating a feed pump or increasing the perfusion rate to influence parameter concentration and maintain a defined steady state.

The BioPAT^® Trace provides continuous parameter analysis with up to one data point per minute during cultivation. It is independent of the type of cultivation (batch, fed-batch, continuous cultivation) and allows the setup of automated parameter control loops. In addition to the online analysis function, scheduled or manually activated parameter calibrations during a batch ensure measurement robustness without stoppage.

BioPAT^® Trace and Multi Trace require a minimum two-point calibration with known traceable parameter solutions to generate the linear measuring range. The measurement frequency of the BioPAT^® Trace filtration probe is up to 60 analyses per hour whereas; the dialysis setup yields 30 analyses per hour from a single vessel. The BioPAT^® Multi Trace must perform additional flushing between vessel sampling and therefore provides analyses every 7 (dialysis) minutes, with four vessels connected. The next sequential data point generated for the first vessel is 28 (dialysis) minutes later. If the required glucose| lactate concentration measured is below 0.1 g/L in dialysis mode the accumulation time increases, resulting in biosensor data points every six minutes. This requires a BioPAT^® Trace firmware update, with an optional change of the dialysis probe membrane to increase the permeability.

The BioPAT^® Trace software can take scheduled samples at any given time point thus increasing or decreasing the sampling frequency to tailor it to specific process control requirements. The ambient temperature of the BioPAT^® Trace can lie between 5°C and 35°C due to internal temperature correction. The ambient humidity should not exceed 90%.

Biobrain^® Supervise digital connection: BioPAT^® Trace and BioPAT^® Multi Trace can be digitally connected by Modbus|OPC^® to Biobrain^® Supervise (OPC DA) and Biobrain^® Supervise (OPC UA) bioprocess management module with additional software from Sartorius Stedim Biotech. This option allows parameter data to be imputed directly into the supervisory control and data aquisition software for all sampled cultivations.