As all living things use O₂ and produce CO₂ the total activity of your cells can be understood from how they are respiring. Therefore, using the combination of a Sartorius Stedim Biostat® bioreactor with mass flow controllers and integrated BioPAT® Xgas you can accurately measure the rate of O₂ uptake (OUR), CO₂ production rate (CPR) and calculate automatically the respiration coefficient (RQ) of your process. These calculations are performed by Biobrain® Supervise which accounts for the dynamic parameter changes of your process conditions. The sensor itself automatically compensates for pressure and humidity so there is no need to have heated exhaust lines. The BioPAT® Xgas fermenter package offers you a powerful process development tool which can reduce your process optimization time by giving clearer online understanding of your cells metabolic activates. Further, with accumulative O₂ uptake or CO₂ production, automated control loops can be established using Biobrain® Supervise S88 recipes which guide your process on a tighter processing pathway giving higher consistency. The BioPAT® Xgas capability of detecting small changes in the percentage of O₂ and CO₂ offer the ability to detect a fault in dissolved O₂ probes and analyze the efficiency of CO₂ stripping. As all of these items come integrated from Sartorius Stedim Biotech they are all tested and covered by our Extend® service coverage.
As all living cells consume O₂ and produce CO₂, the overall activity of your culture can be understood from its respiration behavior. By combining a Sartorius Stedim Biostat® bioreactor with mass flow controllers and the integrated BioPAT® Xgas, you can accurately measure the oxygen uptake rate (OUR) and carbon dioxide production rate (CPR) and automatically calculate the respiratory quotient (RQ) of your process.
These calculations are performed by Biobrain® Supervise, which takes into account dynamic changes in your process conditions. The sensor itself automatically compensates for pressure and humidity, so there is no need for heated exhaust lines.
The BioPAT® Xgas fermenter package provides a powerful process development tool that can shorten your process optimization time by giving a clearer online understanding of your cells’ metabolic activity. Furthermore, with cumulative O₂ uptake or CO₂ production data, automated control loops can be established using Biobrain® Supervise S88 recipes, guiding your process along a tighter operating window and delivering higher consistency.
The high sensitivity of BioPAT® Xgas to small changes in O₂ and CO₂ concentrations also enables early detection of faults in dissolved O₂ probes and supports analysis of CO₂ stripping efficiency.
